Team:Yale/Our Project/Notebook/Week 6

From 2010.igem.org

iGEM Yale

lab notebook: week 6 (7/12-7/18)

  • Monday 7/12--Copper growth assay of pSB74 transformants & continued ligation work
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  • Tuesday 7/13--Transformant Copper growth assay, ligation attempt #4 results, and copper removal assay prep
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  • Wednesday 7/14--Copper Removal assay work and 5th attempt to ligate phsABC into terminator B0015
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  • Thursday 7/15--Confirmation of SpeI activity and EtOH precipitation of ligation components
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  • Friday 7/16--Experimentation with different treatments of vector & insert DNA prior to ligation
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